PoplarV1, Main, Exploration, bibRecord, 000E19

Infrared Nanospectroscopy Reveals the Chemical Nature of Pit Membranes in Water-Conducting Cells of the Plant Xylem.

Identifieur interne : 000E19 ( Main/Exploration ); précédent : 000E18; suivant : 000E20

Infrared Nanospectroscopy Reveals the Chemical Nature of Pit Membranes in Water-Conducting Cells of the Plant Xylem.

Auteurs : Luciano Pereira [Brésil] ; Denisele N A. Flores-Borges [Brésil] ; Paulo R L. Bittencourt [Brésil] ; Juliana L S. Mayer [Brésil] ; Eduardo Kiyota [Brésil] ; Pedro Araújo [Brésil] ; Steven Jansen [Allemagne] ; Raul O. Freitas [Brésil] ; Rafael S. Oliveira [Brésil] ; Paulo Mazzafera [Brésil]

Source :

Plant physiology [ 1532-2548 ] ; 2018.

RBID : pubmed:29871981

Descripteurs français

KwdFr :
- Cellules végétales (composition chimique), Cellules végétales (métabolisme), Cellulose (composition chimique), Eau (métabolisme), Lignine (composition chimique), Membrane cellulaire (composition chimique), Microscopie à force atomique (méthodes), Nanotechnologie (méthodes), Pectine (composition chimique), Populus (cytologie), Spectrophotométrie IR (méthodes), Xylème (cytologie).
MESH :
- composition chimique : Cellules végétales, Cellulose, Lignine, Membrane cellulaire, Pectine.
- cytologie : Populus, Xylème.
- métabolisme : Cellules végétales, Eau.
- méthodes : Microscopie à force atomique, Nanotechnologie, Spectrophotométrie IR.

English descriptors

KwdEn :
- Cell Membrane (chemistry), Cellulose (chemistry), Lignin (chemistry), Microscopy, Atomic Force (methods), Nanotechnology (methods), Pectins (chemistry), Plant Cells (chemistry), Plant Cells (metabolism), Populus (cytology), Spectrophotometry, Infrared (methods), Water (metabolism), Xylem (cytology).
MESH :
- chemical , chemistry : Cellulose, Lignin, Pectins.
- chemistry : Cell Membrane, Plant Cells.
- cytology : Populus, Xylem.
- metabolism : Plant Cells, Water.
- methods : Microscopy, Atomic Force, Nanotechnology, Spectrophotometry, Infrared.

Abstract

In the xylem of angiosperm plants, microscopic pits through the secondary cell walls connect the water-conducting vessels. Cellulosic meshes originated from primary walls, and middle lamella between adjacent vessels, called the pit membrane, separates one conduit from another. The intricate structure of the nano-sized pores in pit membranes enables the passage of water under negative pressure without hydraulic failure due to obstruction by gas bubbles (i.e. embolism) under normal conditions or mild drought stress. Since the chemical composition of pit membranes affects embolism formation and bubble behavior, we directly measured pit membrane composition in Populus nigra wood. Here, we characterized the chemical composition of cell wall structures by synchrotron infrared nanospectroscopy and atomic force microscopy-infrared nanospectroscopy with high spatial resolution. Characteristic peaks of cellulose, phenolic compounds, and proteins were found in the intervessel pit membranes of P. nigra wood. In addition, the vessel to parenchyma pit membranes and developing cell walls of the vascular cambium showed clear signals of cellulose, proteins, and pectin. We did not find a distinct peak of lignin and other compounds in these structures. Our investigation of the complex chemical composition of intervessel pit membranes furthers our understanding of the flow of water and bubbles between neighboring conduits. The advances presented here pave the way for further label-free studies related to the nanochemistry of plant cell components.

DOI: 10.1104/pp.18.00138
PubMed: 29871981
PubMed Central: PMC6084671

Affiliations:

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Le document en format XML

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<term>Nanotechnology (methods)</term>
<term>Pectins (chemistry)</term>
<term>Plant Cells (chemistry)</term>
<term>Plant Cells (metabolism)</term>
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<term>Spectrophotometry, Infrared (methods)</term>
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<term>Membrane cellulaire (composition chimique)</term>
<term>Microscopie à force atomique (méthodes)</term>
<term>Nanotechnologie (méthodes)</term>
<term>Pectine (composition chimique)</term>
<term>Populus (cytologie)</term>
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<term>Cellulose</term>
<term>Lignine</term>
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<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Populus</term>
<term>Xylème</term>
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</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Microscopie à force atomique</term>
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<front><div type="abstract" xml:lang="en">In the xylem of angiosperm plants, microscopic pits through the secondary cell walls connect the water-conducting vessels. Cellulosic meshes originated from primary walls, and middle lamella between adjacent vessels, called the pit membrane, separates one conduit from another. The intricate structure of the nano-sized pores in pit membranes enables the passage of water under negative pressure without hydraulic failure due to obstruction by gas bubbles (i.e. embolism) under normal conditions or mild drought stress. Since the chemical composition of pit membranes affects embolism formation and bubble behavior, we directly measured pit membrane composition in <i>Populus nigra</i>
 wood. Here, we characterized the chemical composition of cell wall structures by synchrotron infrared nanospectroscopy and atomic force microscopy-infrared nanospectroscopy with high spatial resolution. Characteristic peaks of cellulose, phenolic compounds, and proteins were found in the intervessel pit membranes of <i>P. nigra</i>
 wood. In addition, the vessel to parenchyma pit membranes and developing cell walls of the vascular cambium showed clear signals of cellulose, proteins, and pectin. We did not find a distinct peak of lignin and other compounds in these structures. Our investigation of the complex chemical composition of intervessel pit membranes furthers our understanding of the flow of water and bubbles between neighboring conduits. The advances presented here pave the way for further label-free studies related to the nanochemistry of plant cell components.</div>
</front>
</TEI>
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<DateCompleted><Year>2019</Year>
<Month>04</Month>
<Day>23</Day>
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<DateRevised><Year>2019</Year>
<Month>08</Month>
<Day>02</Day>
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<Issue>4</Issue>
<PubDate><Year>2018</Year>
<Month>08</Month>
</PubDate>
</JournalIssue>
<Title>Plant physiology</Title>
<ISOAbbreviation>Plant Physiol</ISOAbbreviation>
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<ArticleTitle>Infrared Nanospectroscopy Reveals the Chemical Nature of Pit Membranes in Water-Conducting Cells of the Plant Xylem.</ArticleTitle>
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<ELocationID EIdType="doi" ValidYN="Y">10.1104/pp.18.00138</ELocationID>
<Abstract><AbstractText>In the xylem of angiosperm plants, microscopic pits through the secondary cell walls connect the water-conducting vessels. Cellulosic meshes originated from primary walls, and middle lamella between adjacent vessels, called the pit membrane, separates one conduit from another. The intricate structure of the nano-sized pores in pit membranes enables the passage of water under negative pressure without hydraulic failure due to obstruction by gas bubbles (i.e. embolism) under normal conditions or mild drought stress. Since the chemical composition of pit membranes affects embolism formation and bubble behavior, we directly measured pit membrane composition in <i>Populus nigra</i>
 wood. Here, we characterized the chemical composition of cell wall structures by synchrotron infrared nanospectroscopy and atomic force microscopy-infrared nanospectroscopy with high spatial resolution. Characteristic peaks of cellulose, phenolic compounds, and proteins were found in the intervessel pit membranes of <i>P. nigra</i>
 wood. In addition, the vessel to parenchyma pit membranes and developing cell walls of the vascular cambium showed clear signals of cellulose, proteins, and pectin. We did not find a distinct peak of lignin and other compounds in these structures. Our investigation of the complex chemical composition of intervessel pit membranes furthers our understanding of the flow of water and bubbles between neighboring conduits. The advances presented here pave the way for further label-free studies related to the nanochemistry of plant cell components.</AbstractText>
<CopyrightInformation>© 2018 American Society of Plant Biologists. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pereira</LastName>
<ForeName>Luciano</ForeName>
<Initials>L</Initials>
<Identifier Source="ORCID">0000-0003-2225-2957</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Biology, Institute of Biology, University of Campinas, 13083-970 Campinas, Sao Paulo, Brazil.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Flores-Borges</LastName>
<ForeName>Denisele N A</ForeName>
<Initials>DNA</Initials>
<Identifier Source="ORCID">0000-0002-8500-3205</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Biology, Institute of Biology, University of Campinas, 13083-970 Campinas, Sao Paulo, Brazil.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bittencourt</LastName>
<ForeName>Paulo R L</ForeName>
<Initials>PRL</Initials>
<Identifier Source="ORCID">0000-0002-1618-9077</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Biology, Institute of Biology, University of Campinas, 13083-970 Campinas, Sao Paulo, Brazil.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mayer</LastName>
<ForeName>Juliana L S</ForeName>
<Initials>JLS</Initials>
<Identifier Source="ORCID">0000-0001-7439-5234</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Biology, Institute of Biology, University of Campinas, 13083-970 Campinas, Sao Paulo, Brazil.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kiyota</LastName>
<ForeName>Eduardo</ForeName>
<Initials>E</Initials>
<Identifier Source="ORCID">0000-0001-6322-3461</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Biology, Institute of Biology, University of Campinas, 13083-970 Campinas, Sao Paulo, Brazil.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Araújo</LastName>
<ForeName>Pedro</ForeName>
<Initials>P</Initials>
<Identifier Source="ORCID">0000-0002-3205-5749</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Biology, Institute of Biology, University of Campinas, 13083-970 Campinas, Sao Paulo, Brazil.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jansen</LastName>
<ForeName>Steven</ForeName>
<Initials>S</Initials>
<Identifier Source="ORCID">0000-0002-4476-5334</Identifier>
<AffiliationInfo><Affiliation>Ulm University, Institute of Systematic Botany and Ecology, 89081 Ulm, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Freitas</LastName>
<ForeName>Raul O</ForeName>
<Initials>RO</Initials>
<Identifier Source="ORCID">0000-0002-3285-5447</Identifier>
<AffiliationInfo><Affiliation>Brazilian Synchrotron Light Laboratory, Brazilian Center for Research in Energy and Materials, 13083-970 Campinas, Sao Paulo, Brazil.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Oliveira</LastName>
<ForeName>Rafael S</ForeName>
<Initials>RS</Initials>
<Identifier Source="ORCID">0000-0002-6392-2526</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Biology, Institute of Biology, University of Campinas, 13083-970 Campinas, Sao Paulo, Brazil.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mazzafera</LastName>
<ForeName>Paulo</ForeName>
<Initials>P</Initials>
<Identifier Source="ORCID">0000-0001-9073-4056</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Biology, Institute of Biology, University of Campinas, 13083-970 Campinas, Sao Paulo, Brazil pmazza@unicamp.br.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Crop Production, School of Agriculture Luiz de Queiroz, University of São Paulo, 13418-900 Piracicaba, Sao Paulo, Brazil.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2018</Year>
<Month>06</Month>
<Day>05</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Plant Physiol</MedlineTA>
<NlmUniqueID>0401224</NlmUniqueID>
<ISSNLinking>0032-0889</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>059QF0KO0R</RegistryNumber>
<NameOfSubstance UI="D014867">Water</NameOfSubstance>
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<Chemical><RegistryNumber>89NA02M4RX</RegistryNumber>
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<Chemical><RegistryNumber>9004-34-6</RegistryNumber>
<NameOfSubstance UI="D002482">Cellulose</NameOfSubstance>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D002462" MajorTopicYN="N">Cell Membrane</DescriptorName>
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</MeshHeading>
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<MeshHeading><DescriptorName UI="D059828" MajorTopicYN="N">Plant Cells</DescriptorName>
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<MeshHeading><DescriptorName UI="D052584" MajorTopicYN="N">Xylem</DescriptorName>
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Infrared Nanospectroscopy Reveals the Chemical Nature of Pit Membranes in Water-Conducting Cells of the Plant Xylem.

Infrared Nanospectroscopy Reveals the Chemical Nature of Pit Membranes in Water-Conducting Cells of the Plant Xylem.

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